On-line monitoring of bioprocesses is an important tool in production, process development and R&D and is fundamental for process control. Its foremost advantage is the monitoring of sudden changes in critical process parameters by a virtually unlimited sample number. On-line cell density monitoring reduces the risk of missing important events which would remain undetected if classical on-line biomass measurement techniques would be used like for example the calculation of the oxygen uptake rate (OUR) or the CO2 evolution rate (CER).
One of the most important parameters to monitor in any cell culture process is the viable, that is, metabolically active cell volume.
Hamilton’s cell density sensors measure viable cell volume based on capacitance. The measuring line consists of an Incyte or Dencytee Sensor, an Incyte Pre-Amp and an Arc View transmitter. The Arc View transmitter integrates the measurements of viable and total cell density, pH and dissolved oxygen measurement in one device and can be used as stand-alone device, as well as being integrated into a process control system.
One of the most important parameters to monitor in any cell culture process is the viable, that is, metabolically active cell volume.
Hamilton’s cell density sensors measure viable cell volume based on capacitance. The measuring line consists of an Incyte or Dencytee Sensor, an Incyte Pre-Amp and an Arc View transmitter. The Arc View transmitter integrates the measurements of viable and total cell density, pH and dissolved oxygen measurement in one device and can be used as stand-alone device, as well as being integrated into a process control system.
Key benefits:
· Characterisation of process dynamics support understanding of the cell culture.
The measured parameters of the capacitance sensors are linked to biovolume, intracellular conductivity, membrane capacitance and cell size distribution and allow more precise declaration on the cells/process compared to the optical density probe.
· Optimisation of critical parameters, like feeding strategy, inoculation and harvesting time
· Early detection of process deviations allows better cell culture traceability.
· Reduction of contamination risks which are generally increased by frequent sampling
· Arc View transmitter allows the direct correlation of viable cell volume with process controlling parameters like pH and DO
Results:
· Higher production yields
· Consistent product quality
· Improved productivity
· Cost savings: Reduced man hours for off-line sampling methods
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· Characterisation of process dynamics support understanding of the cell culture.
The measured parameters of the capacitance sensors are linked to biovolume, intracellular conductivity, membrane capacitance and cell size distribution and allow more precise declaration on the cells/process compared to the optical density probe.
· Optimisation of critical parameters, like feeding strategy, inoculation and harvesting time
· Early detection of process deviations allows better cell culture traceability.
· Reduction of contamination risks which are generally increased by frequent sampling
· Arc View transmitter allows the direct correlation of viable cell volume with process controlling parameters like pH and DO
Results:
· Higher production yields
· Consistent product quality
· Improved productivity
· Cost savings: Reduced man hours for off-line sampling methods
CLICK HERE for more information
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